The transfer of frozen embryos is scheduled so that the uterine environment is favorable to receive the embryo, and have an optimal implantation period. The preparation of the endometrium happens either during a natural cycle, or a pharmaceutical cycle. The embryos are usually thawed a few hours before the embryo transfer.

After thawing, the number of embryos and blastomeres that have survived are evaluated. Embryo quality is assessed and the number of embryos for transfer is then decided.